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Mail sol. C14

Internal Solution-ID: S6005

This solution is used in 2 media and was first defined in medium C14.

Compound Amount Unit Conc. [g/L] Conc. [mM]
NaNO3
(75 g/l stock solution)
10 ml - -
CaCl2 x 2 H2O
(2.5 g/l stock solution)
10 ml - -
MgSO4 x 7 H2O
(7.5 g/l stock solution)
10 ml - -
K2HPO4 x 3 H2O
(7.5 g/l stock solution)
10 ml - -
KH2PO4
(17.5 g/l stock solution)
10 ml 23.4 171.951
NaCl
(2.5 g/l stock solution)
10 ml - -
Trace Elements (PIV) 6 ml - -
1 Once elements are dissolved autoclave at 15 psi for 15 minutes.
Vitamin B1 solution 1 ml - -
Vitamin B12 solution 1 ml - -
Agar , for solid medium 15 g 15 -
2 Make up to 1 litre with distilled water. For agar add 15 g per litre Bacterialogical Agar. Autoclave at 15 psi for 15 minutes.
C14 3N-BBM+V
C89 SBBM