Internal Solution-ID: S6005
This solution is used in 2 media and was first defined in medium C14.
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
NaNO3
(75 g/l stock solution) |
10 | ml | - | - | |
|
CaCl2 x 2 H2O
(2.5 g/l stock solution) |
10 | ml | - | - | |
|
MgSO4 x 7 H2O
(7.5 g/l stock solution) |
10 | ml | - | - | |
|
K2HPO4 x 3 H2O
(7.5 g/l stock solution) |
10 | ml | - | - | |
|
KH2PO4
(17.5 g/l stock solution) |
10 | ml | 23.4 | 171.951 | |
|
NaCl
(2.5 g/l stock solution) |
10 | ml | - | - | |
| Trace Elements (PIV) | 6 | ml | - | - | |
| 1 Once elements are dissolved autoclave at 15 psi for 15 minutes. | |||||
| Vitamin B1 solution | 1 | ml | - | - | |
| Vitamin B12 solution | 1 | ml | - | - | |
| Agar , for solid medium | 15 | g | 15 | - | |
| 2 Make up to 1 litre with distilled water. For agar add 15 g per litre Bacterialogical Agar. Autoclave at 15 psi for 15 minutes. | |||||