Main sol. 326
Internal Solution-ID: S601
Equipment needed: Hungate tubes
Filter
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 3.30 | g | 3.293 | 24.201 | |
| MgCl2 x 6 H2O | 0.16 | g | 0.16 | 0.785 | |
| CoCl2 x 6 H2O | 12.00 | mg | 0.012 | 0.092 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| Yeast extract | 2.00 | g | 1.996 | - | |
|
Trypticase peptone
(BD BBL) |
2.00 | g | 1.996 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
| Na2CO3 | 1.00 | g | 0.998 | 9.416 | |
| Sucrose | 5.00 | g | 4.99 | 14.578 | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.30 | g | 0.299 | 1.705 | |
| Na2S x 9 H2O | 0.30 | g | 0.299 | 1.247 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except carbonate, sucrose, vitamins, cysteine and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add sucrose, cysteine and sulfide to the medium from sterile stock solutions autoclaved under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins are prepared under 100% N2 gas and sterilized by filtration. Adjust pH of complete medium to pH 6.8 - 7.2, if necessary. | |||||
Media with this solution
| 326 | THERMOCLOSTRIDIUM (A-GÖ) MEDIUM |