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Mail sol. C47

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Internal Solution-ID: S6038

Compound Amount Unit Conc. [g/L] Conc. [mM]
Tris 1 g 1 8.255
NaCl 30 g 30 513.347
MgSO4 x 7 H2O
(24.4 g/100 ml stock solution) 		10 ml - -
KCl
(6 g/100 ml stock solution) 		10 ml - -
NaNO3
(10 g/100 ml stock solution) 		10 ml - -
CaCl2 x 2 H2O
(3 g/100 ml stock solution) 		10 ml - -
KH2PO4
(0.5 g/100 ml stock solution) 		10 ml 23.4 171.951
NH4Cl
(2.67 g/100 ml stock solution) 		1 ml 1.527 28.555
Trace Elements (PIV) 6 ml - -
Vitamin B1 solution 1 ml - -
Vitamin B12 solution 1 ml - -
SE2 (Soil Extract 2) 30 ml - -
1 Make up to 1 litre with distilled water and adjust to pH 8.0 with 1M NaOH or 1M HCl. Autoclave at 15 psi for 15 minutes.
2 For brackish organisms, take 15 g of NaCl instead of 30 g (BW/MASM).
C47 MASM