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Vitamin mix
Internal Solution-ID: S6161
This solution is used in 5 media and was first defined in medium C34.
| 1 First make primary stocks of Cyanocobalamin and Biotin. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Cyanocobalamine
(0.01 g/100 ml stock solution) |
1.00 | ml | - | - | |
|
Biotin
(0.01 g/100 ml stock solution) |
1.00 | ml | - | - | |
| 2 Dispense any excess primary stocks into 1 ml aliquots and freeze. | |||||
| Thiamine HCl | 0.02 | g | 0.1 | 0.296 | |