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Main sol. 48

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Internal Solution-ID: S64

Equipment needed: Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 5.45 g 5.45 31.29
KH2PO4 1.20 g 1.2 8.818
MgSO4 x 7 H2O 25.00 mg 0.025 0.101
CaCl2 x 2 H2O 15.00 mg 0.015 0.102
FeSO4 x 7 H2O 10.00 mg 0.01 0.036
MnCl2 x 4 H2O 2.00 mg 0.002 0.01
CoCl2 x 6 H2O 2.50 mg 0.003 0.019
Na2MoO4 x 2 H2O 2.50 mg 0.003 0.01
Glucose 20.00 g 20 111.012
Peptone 2.00 g 2 -
Tryptone 2.00 g 2 28.137
Yeast extract 6.00 g 6 -
Na-thioglycolate 0.50 g 0.5 4.382
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except glucose), adjust pH to 7.5 and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave.
2 Add glucose to the medium after autoclaving from a sterile stock solution prepared under 100% N2 gas.
48 TRYPTONE THIOGLYCOLATE MEDIUM