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Main sol. 338

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Internal Solution-ID: S650

Equipment needed: Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Beef extract
(Difco) 		3.00 g 3 -
Trypticase
(BBL) 		9.00 g 9 -
Yeast extract 11.00 g 11 -
NaCl 2.00 g 2 34.223
Na2HPO4 0.40 g 0.4 2.818
Na2CO3 0.25 g 0.25 2.359
Haemin 5.00 mg 0.005 0.008
Na-formate 2.00 g 2 29.409
Na-fumarate 3.00 g 3 18.746
Resazurin 1.00 mg 1.0e-3 0.004
Distilled water 1000.00 ml - -
1 Adjust pH to 7.5. Prepare the medium anaerobically under 100% N2.
2 Autoclave solutions of haemin (dissolved in 0.1 ml 1N NaOH, then diluted with water to 10 ml and neutralized), formate, and fumarate separately under nitrogen.
338 CAMPYLOBACTER RECTUS MEDIUM