Main sol. 339
Internal Solution-ID: S651
Equipment needed: Filter
Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
dehydrated Wilkins-Chalgren medium
(Oxoid CM0643) |
33.0 | g | 33 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
| L-Cysteine HCl | 0.3 | g | 0.3 | 1.903 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve 33.0 g/l dehydrated Wilkins-Chalgren medium (Oxoid CM0643) in distilled water and add 0.5 ml/l Na-resazurin solution (0.1% w/v), bring to the boil and cool to room temperature while sparging with 100% N2 gas. Add L-cysteine-HCL x H2O (0.3 g/l), dispense the medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving check pH and adjust to pH 6.8 - 7.0, if necessary. | |||||
| 2 Note: In case resazurin is not colourless addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) can be used to completely reduce the medium. | |||||
Media with this solution
| 339 | WILKINS-CHALGREN ANAEROBE BROTH |