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Solution A

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Internal Solution-ID: S655

Equipment needed: Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 1.0 g 1.263 23.605
MgCl2 x 6 H2O 0.1 g 0.126 0.621
CaCl2 x 2 H2O 0.1 g 0.126 0.859
K2HPO4 0.4 g 0.505 2.9
Na2SeO3 x 5 H2O
(0.01% w/v) 		2.0 ml 2.5e-4 9.6e-4
Modified Wolin's mineral solution 10.0 ml - -
Yeast extract
(OXOID) 		2.0 g 2.525 -
Trypticase peptone
(BD BBL) 		2.0 g 2.525 -
Sodium resazurin
(0.1% w/v) 		0.5 ml 6.3e-4 0.003
L-Cysteine HCl x H2O 0.5 g 0.631 3.594
Distilled water 780.0 ml - -
342 METHANOBACTERIUM ALCALIPHILUM MEDIUM