Main sol. 343
Internal Solution-ID: S658
Equipment needed: Autoclave
Hungate tubes
Gassing station
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Starch
(soluble) |
5.0 | g | 4.965 | - | |
| KH2PO4 | 0.5 | g | 0.497 | 3.649 | |
| Modified Wolin's mineral solution | 15.0 | ml | - | - | |
| Artificial sea water | 250.0 | ml | - | - | |
|
Yeast extract
(OXOID) |
2.0 | g | 1.986 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
| Wolin's vitamin solution (10x) | 1.0 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.497 | 2.827 | |
| Na2S x 9 H2O | 0.5 | g | 0.497 | 2.067 | |
| Distilled water | 740.0 | ml | - | - | |
| 1 Dissolve ingredients (except vitamins, sulfide, and cysteine) and adjust pH to 6.5. Boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere. Dispense under the same gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of volume and autoclave. Add vitamins, sulfide, and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas. Sterilize vitamins by filtration. Adjust the pH of the complete medium to 6.5, if necessary. | |||||
Media with this solution
| 343 | THERMOTOGA NEAPOLITANA MEDIUM |