Main sol. 343a
Internal Solution-ID: S660
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Starch
(soluble) |
5.0 | g | 5 | - | |
| KH2PO4 | 0.5 | g | 0.5 | 3.674 | |
| Modified Wolin's mineral solution | 15.0 | ml | - | - | |
|
NiCl2 x 6 H2O
(0.1% w/v) |
2.0 | ml | 0.002 | 0.015 | |
| NaCl | 20.0 | g | 20 | 342.231 | |
| Artificial sea water | 250.0 | ml | - | - | |
|
Yeast extract
(OXOID) |
2.0 | g | 2 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | - | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.5 | 2.847 | |
| Na2S x 9 H2O | 0.5 | g | 0.5 | 2.082 | |
| Distilled water | 750.0 | ml | - | - | |
| 1 Dissolve ingredients (except sulfide and cysteine) and adjust pH to 6.5. Boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere. Dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of volume and autoclave. Add sulfide and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas. Adjust pH of complete medium to 6.5, if necessary. | |||||
Media with this solution
| 343a | THERMOSIPHO MELANESIENSIS MEDIUM |