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Main sol. 346

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Internal Solution-ID: S662

Equipment needed: Gassing station Hungate tubes Autoclave Filter Centrifuge
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 966.0 ml - -
Solution B 20.0 ml - -
Solution C 11.0 ml - -
Solution D 10.0 ml - -
Solution E 1.0 ml - -
Solution F 1.0 ml - -
Solution G 1.0 ml - -
Solution H 1.7 ml - -
1 Sparge solution A with 100% N2 gas for 30 - 45 min to make it anoxic. Thereafter, dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Anoxic stock solutions C, D, E, F, and H are prepared under 100% N2 gas atmosphere and solution G under 80% N2 and 20% CO2 gas atmosphere. Filter sterilize solutions D, F and G. To complete the medium appropriate amounts of solutions B to H are added to the sterile solution A in the sequence as indicated. Adjust pH of the complete medium to 7.2 - 7.5, if necessary.
346 DESULFALLAS MEDIUM