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Solution A

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Internal Solution-ID: S672

Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.50 g 0.543 3.989
MgCl2 x 6 H2O 0.33 g 0.358 1.762
NaCl 0.40 g 0.434 7.432
NH4Cl 0.40 g 0.434 8.119
CaCl2 x 2 H2O 0.05 g 0.054 0.369
Trace element solution SL-10 1.00 ml - -
Clarified rumen fluid 50.00 ml - -
Trypticase peptone
(BD BBL) 		1.00 g 1.086 -
PIPES
(SIGMA) 		15.00 g 16.287 53.863
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.4e-4 0.002
Distilled water 870.00 ml - -
347 SYNTROPHOMONAS SAPOVORANS MEDIUM