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500 mM Fe(III)NTA Stock solution

Internal Solution-ID: S7

Equipment needed: Filter Anaerobic workbench
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 8.2 g 117.143 1394.45
2 70.0 ml 998.207 55408.8
3 12.8 g 182.857 -
4 13.5 g 192.857 713.503
5 10.0 N - -
Instructions:
  1. Add 8.2 g NaHCO3 to ca. 70 ml distilled water. Add 12.8 g of Na3Nitrilotriacetic acid ( NTA) to ca. 70 ml distilled water. Add 13.5 g FeCl3 x 6 H2O to ca 70 ml distilled water. Adjust pH to 6.5 using 10N NaOH. Bring solution to final volume of 100 ml. Ingredients will go into solution after stirring for ca. 15 min. Bubble with anaerobic gas (N2) for 45 min and then filter sterilize (0.2 μm filter) into a sterile, anaerobic, serum bottle.
1001 BASAL MEDIUM