Main sol. 373
Internal Solution-ID: S713
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KCl | 0.34 | g | 0.34 | 4.556 | |
MgCl2 x 6 H2O | 4.00 | g | 3.996 | 19.655 | |
MgSO4 x 7 H2O | 3.45 | g | 3.447 | 13.983 | |
NH4Cl | 0.25 | g | 0.25 | 4.669 | |
CaCl2 x 2 H2O | 0.14 | g | 0.14 | 0.951 | |
K2HPO4 | 0.14 | g | 0.14 | 0.803 | |
NaCl | 18.00 | g | 17.982 | 307.701 | |
Modified Wolin's mineral solution | 10.00 | ml | - | - | |
Fe(NH4)2(SO4)2 x 6 H2O
(0.1% w/v) |
2.00 | ml | 0.002 | - | |
Na-acetate | 1.00 | g | 0.999 | 12.178 | |
Yeast extract
(OXOID) |
2.00 | g | 1.998 | - | |
Trypticase peptone
(BD BBL) |
2.00 | g | 1.998 | - | |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
NaHCO3 | 5.00 | g | 4.995 | 59.459 | |
Isopropyl alcohol | 7.50 | ml | - | - | |
Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
Na2S x 9 H2O | 0.50 | g | 0.5 | 2.08 | |
Distilled water | 980.00 | ml | - | - | |
1 Dissolve ingredients (except bicarbonate, isopropyl alcohol, vitamins and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add and dissolve bicarbonate and adjust pH to 7.0, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add isopropyl alcohol and cysteine from sterile anoxic stock solutions autoclaved under 100% N2 gas atmosphere. Vitamins are prepared under 100% N2 gas atmosphere and sterilized by filtration. Adjust pH of complete medium to 6.8 - 7.0, if necessary. |
373 | METHANOGENIUM CV MEDIUM |