Main sol. 380a
Internal Solution-ID: S724
Equipment needed: Autoclave
Hungate tubes
Filter
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.68 | g | 0.675 | 4.957 | |
| NaNO3 | 0.12 | g | 0.119 | 1.401 | |
| L(+)-Tartaric acid | 0.37 | g | 0.367 | 2.446 | |
| Succinic acid | 0.37 | g | 0.367 | 3.108 | |
| Na-acetate | 0.05 | g | 0.05 | 0.605 | |
| Modified Wolin's mineral solution | 5.00 | ml | - | - | |
| Fe(III) quinate solution 0.01 M | 2.00 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
| D-Xylose | 1.00 | g | 0.992 | 6.608 | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
| Na-thioglycolate | 0.05 | g | 0.05 | 0.435 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except xylose, vitamins and thioglycolate) and adjust pH to 5.5 - 6.0. Sparge medium with 100% N2 gas for 30 -45 min to make it anoxic and dispense under the same gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume. Seal vials with gas tight butyl rubber closures. Autoclave at 121°C for 15 min. Before inoculation add xylose, vitamins and thioglycolate from anoxic stock solutions prepared under 100% N2 gas and sterilized by filtration. Then add sterile air (with hypodermic syringe through the rubber closure) to 1% (v/v) O2 concentration in the gas phase. | |||||
Media with this solution
| 380a | TELMATOSPIRILLUM (PEAT) MEDIUM |