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Main sol. 386

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Internal Solution-ID: S778

Equipment needed: Gassing station Hungate tubes Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 942 ml - -
Solution B 30 ml - -
Solution C 10 ml - -
Solution D 1 ml - -
Solution E 10 ml - -
Solution F 13 ml - -
1 Solution A is sparged 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic, then distributed under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclaved. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas mixture. Solutions C and F are autoclaved under 100% N2 gas. Solutions D and E are prepared under 100% N2 gas atmosphere and sterilized by filtration. To complete the medium appropriate amounts of the solutions B to F are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be at 7.1 - 7.4.
2 Note: When growth has started feed culture again with same amount of pyrosulfite. After a further two days repeat feeding once more.
386 DESULFOLUTIVIBRIO MEDIUM