Main sol. 391
Internal Solution-ID: S799
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Mineral solution 2xW | 500.0 | ml | - | - | |
| Modified Wolin's mineral solution | 10.0 | ml | - | - | |
| LIP-solution | 50.0 | ml | - | - | |
| Na-acetate | 1.0 | g | 1 | 12.19 | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | - | |
| TYC-solution | 50.0 | ml | - | - | |
| Na2CO3 | 1.5 | g | 1.5 | 14.153 | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.5 | 2.847 | |
| Na2S x 9 H2O | 0.5 | g | 0.5 | 2.082 | |
| Distilled water | 400.0 | ml | - | - | |
| 1 Dissolve ingredients except TYC solution, carbonate, cystein and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Before use add TYC solution, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of complete medium to 7.2, if necessary. | |||||
Media with this solution
| 391 | METHANOCOCCUS VOLTAE MEDIUM |