Main sol. 396
Internal Solution-ID: S809
Equipment needed: Hungate tubes
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Yeast extract
(OXOID) |
2.00 | g | 2 | - | |
|
Trypticase peptone
(BD BBL) |
2.00 | g | 2 | - | |
| NaCl | 40.00 | g | 40 | 684.463 | |
| NH4Cl | 1.00 | g | 1 | 18.695 | |
| MgCl2 x 6 H2O | 3.50 | g | 3.5 | 17.216 | |
| MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
| KCl | 1.00 | g | 1 | 13.414 | |
| K2HPO4 | 0.40 | g | 0.4 | 2.297 | |
| Modified Wolin's mineral solution | 5.00 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.5 | 2.847 | |
| NaHCO3 | 3.00 | g | 3 | 35.711 | |
| Na2CO3 | 2.00 | g | 2 | 18.87 | |
| Trimethylamine-HCl | 2.00 | g | 2 | - | |
| Na2S x 9 H2O | 0.25 | g | 0.25 | 1.041 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients except cysteine, bicarbonate, carbonate, trimethylamine and sulfide, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add cysteine and dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add trimethylamine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas, whereas bicarbonate and carbonate are added from sterile anoxic stock solutions prepared under 80% N2 and 20% CO2 gas atmosphere. Adjust pH of complete medium to 9.2 - 9.4. | |||||
Media with this solution
| 396 | METHANOSALSUM ZHILINAE MEDIUM |