1
Combine the warm (37°C) solutions A, B, C, D, and filter-sterilize the warm medium. Solution B can also be added after filtration.
2
Alternatively, buy supplemented or un-supplemented BSK-H medium (Fa. Bio&SELL or Sigma) and supplement the medium according to manufacturer's advice. Sterilize the mixed and warm (37°C) medium by filtration.
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