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Main sol. 405

Internal Solution-ID: S826

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Casitone
(Difco)
1.00 g 1 -
Yeast extract
(Difco)
0.20 g 0.2 -
Na-acetate 0.02 g 0.02 0.244
Agar , if required
(Difco)
15.00 g 15 44.599
Artificial sea water OR Sea water 1000.00 ml - -
1 Adjust pH to 7.2.
2 If natural sea water is used, the pH need not be adjusted.
405 SP2 MEDIUM