Main sol. 408
Internal Solution-ID: S835
Equipment needed: Hungate tubes
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Peptone
(BD Bacto) |
10.0 | g | 10 | - | |
|
Yeast extract
(OXOID) |
3.0 | g | 3 | - | |
|
Casamino acids
(BD Bacto) |
10.0 | g | 10 | - | |
| L-Glutamic acid | 20.0 | g | 20 | 135.934 | |
| NH4Cl | 2.0 | g | 2 | 37.39 | |
| K2HPO4 x 3 H2O | 2.0 | g | 2 | 11.483 | |
| NaCl | 25.0 | g | 25 | 427.789 | |
| Modified Wolin's mineral solution | 10.0 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | - | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.5 | 2.847 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients (except cysteine), bring medium to the boil, then cool to room temperature under 100% N2 gas atmosphere. Add and dissolve cysteine, adjust pH to 7.0 and dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. | |||||
Media with this solution
| 408 | PSYCHRILYOBACTER SP. MEDIUM |