Main sol. 412
Internal Solution-ID: S842
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Trypticase peptone | 2.000 | g | 2 | - | |
| Yeast extract | 2.000 | g | 2 | - | |
| Glucose | 4.000 | g | 4 | 22.203 | |
| K2HPO4 | 0.225 | g | 0.225 | 1.292 | |
| KH2PO4 | 0.255 | g | 0.255 | 1.874 | |
| (NH4)2SO4 | 0.255 | g | 0.255 | 1.93 | |
| NaCl | 0.500 | g | 0.5 | 8.556 | |
| MgSO4 x 7 H2O | 0.100 | g | 0.1 | 0.406 | |
| CaCl2 x 2 H2O | 0.070 | g | 0.07 | 0.476 | |
| Na-acetate | 5.000 | g | 5 | 60.951 | |
| Wolin's vitamin solution | 10.000 | ml | - | - | |
| Modified Wolin's mineral solution | 10.000 | ml | - | - | |
| Resazurin | 1.000 | mg | 1.0e-3 | 0.004 | |
| NaHCO3 | 6.000 | g | 6 | 71.423 | |
| L-Cysteine HCl x H2O | 0.500 | g | 0.5 | 2.847 | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 Autoclave under 2 bar N2:CO2 (80:20). | |||||
Media with this solution
| 412 | ACETOMICROBIUM FAECALIS MEDIUM |