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Main sol. 429d

Internal Solution-ID: S872

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Columbia agar base
(dissolved in 70 % artificial seawater)
1000.0 ml 700 -
Agar 2.0 g/l 2 5.947
Horse blood OR Sheep blood 4.0 % 40 -
Glucose 1.0 % 10 55.506
FeCl3 2.0 mM 0.324 2
Cysteine 0.1 % 1 8.254
1 Dissolve Columbia agar base in 70% artificial seawater and add additional 2 g agar per liter. Autoclave and mix solution with 4% horse or sheep blood after cooling to 50°C. Add 1% glucose, 2 mM FeCl3 and 0.1% cysteine, mix well and pour plates. Rehydrate pellets of freeze-dried ampoules in liquid medium 220 or 215.
429d FRANCISELLA HALIOTICIDA MEDIUM