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Lactic acid whey

Internal Solution-ID: S911

Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Skim milk 10 % 100 -
1 Adjust 10% skim milk to pH 5.5 with lactic acid and heat at 100°C for 30 minutes. Remove precipitate by filtration. For preparing broth medium, adjust filtrate to pH 7.0 with 2 N NaOH and heat at 100°C for 30 minutes. Remove precipitate by filtration. Adjust filtrate to pH 5.5 with 2 N HCl.
456 KPL MEDIUM