Main sol. 465i
Internal Solution-ID: S957
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
2-chloro-4(ethylamino)-6-(isopropylamino)-1,3,5-triazine
, adjust if required
(suspend 10 mg/ml in methanol) |
100 | mg/l | - | - | |
Medium 465 | 1000 | ml | - | - | |
1 Suspend atrazine (2-chloro-4(ethylamino)-6-(isopropylamino)-1,3,5-triazine) in methanol (10 mg/ml). Shortly sonicate to reduce particle size. Add stock suspension to medium 465 without nitrogen source (omit ammonium sulphate and calcium nitrate), replace calcium nitrate by calcium chloride) but with 1 g/l glucose as the carbon source. Final concentration of atrazine is 50-100 mg/l. |
465i | MINERAL MEDIUM WITH ATRAZIN |