Main sol. 479
Internal Solution-ID: S976
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 87.00 | g | 87 | 1488.71 | |
KCl | 1.50 | g | 1.5 | 20.121 | |
MgCl2 x 6 H2O | 6.00 | g | 6 | 29.513 | |
CaCl2 x 2 H2O | 0.40 | g | 0.4 | 2.721 | |
NH4Cl | 1.00 | g | 1 | 18.695 | |
K2HPO4 x 3 H2O | 0.40 | g | 0.4 | 2.297 | |
Modified Wolin's mineral solution | 10.00 | ml | - | - | |
Yeast extract
(OXOID) |
2.00 | g | 2 | - | |
Trypticase peptone
(BD BBL) |
2.00 | g | 2 | - | |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
Na2CO3 | 1.50 | g | 1.5 | 14.153 | |
Trimethylamine-HCl | 2.00 | g | 2 | - | |
2-Mercaptoethanesulfonic acid
(coenzyme M) |
0.20 | g | 0.2 | 1.406 | |
Na2S x 9 H2O | 0.25 | g | 0.25 | 1.041 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients except carbonate, trimethylamine, coenzyme M and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add trimethylamine, coenzyme M and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of complete medium to 7.0 - 7.2, if necessary. |
479 | METHANOHALOPHILUS MEDIUM |