Main sol. 479a
Internal Solution-ID: S977
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 120.00 | g | 120 | 2053.39 | |
| KCl | 1.50 | g | 1.5 | 20.121 | |
| MgCl2 x 6 H2O | 6.00 | g | 6 | 29.513 | |
| CaCl2 x 2 H2O | 0.40 | g | 0.4 | 2.721 | |
| NH4Cl | 1.00 | g | 1 | 18.695 | |
| K2HPO4 x 3 H2O | 0.40 | g | 0.4 | 2.297 | |
| Modified Wolin's mineral solution | 10.00 | ml | - | - | |
|
Yeast extract
(OXOID) |
2.00 | g | 2 | - | |
|
Trypticase peptone
(BD BBL) |
2.00 | g | 2 | - | |
|
HEPES
(SIGMA H3375) |
5.00 | g | 5 | 20.982 | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
| Trimethylamine-HCl | 2.00 | g | 2 | - | |
|
2-Mercaptoethanesulfonic acid
(coenzyme M) |
0.20 | g | 0.2 | 1.406 | |
| Na2S x 9 H2O | 0.25 | g | 0.25 | 1.041 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients except trimethylamine, coenzyme M and sulfide. Adjust pH to 7.5 and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic; then dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add trimethylamine, coenzyme M and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Adjust pH of complete medium to 7.5, if necessary. | |||||
Media with this solution
| 479a | METHANOHALOPHILUS PROFUNDI MEDIUM |