Main sol. 480
Internal Solution-ID: S978
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NH4Cl | 0.33 | g | 0.33 | 6.169 | |
CaCl2 x 2 H2O | 0.33 | g | 0.33 | 2.245 | |
MgCl2 x 6 H2O | 0.33 | g | 0.33 | 1.623 | |
KCl | 0.33 | g | 0.33 | 4.427 | |
KH2PO4 | 0.33 | g | 0.33 | 2.425 | |
Trace element solution SL-10 | 1.00 | ml | - | - | |
Yeast extract | 0.10 | g | 0.1 | - | |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
Sulfur
(powder) |
10.00 | g | 10 | 311.818 | |
Na-acetate | 5.00 | g | 5 | 60.951 | |
NaHCO3 | 2.50 | g | 2.5 | 29.759 | |
Wolin's vitamin solution | 10.00 | ml | - | - | |
Na2S x 9 H2O | 0.50 | g | 0.5 | 2.082 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients (except sulfur, acetate, bicarbonate, vitamins and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Adjust pH to 5.9, dispense under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials containing already the appropriate amount of sulfur and sterilize by heating for 2-3 hours in a boiling water bath on each of 3 successive days. Add acetate, vitamins (sterilized by filtration)and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 6.8 - 7.0, if necessary. |
480d | THERMOPROTEUS UZONIENSIS MEDIUM |