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Main sol. 482

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Internal Solution-ID: S984

Equipment needed: Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.33 g 0.33 2.425
NH4Cl 0.33 g 0.33 6.169
MgCl2 x 6 H2O 0.33 g 0.33 1.623
CaCl2 x 2 H2O 0.33 g 0.33 2.245
KCl 0.33 g 0.33 4.427
Trace element solution SL-10 1.00 ml - -
Yeast extract 1.00 g 1 -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 -
NaHCO3 1.50 g 1.5 17.856
Sucrose 2.00 g 2 5.843
L-Cysteine HCl x H2O 0.30 g 0.3 1.708
Na2S x 9 H2O 0.30 g 0.3 1.249
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except bicarbonate, sucrose, cysteine and sulfide), adjust pH to 7.0, and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add sucrose, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of complete medium to pH 7.0, if necessary.
482 ANAEROBACTER MEDIUM