Main sol. 573
Internal Solution-ID: S1236
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NH4Cl | 0.20 | g | 0.2 | 3.739 | |
K2HPO4 | 0.01 | g | 0.01 | 0.057 | |
MgSO4 x 7 H2O | 0.01 | g | 0.01 | 0.041 | |
CaSO4
(saturated solution) |
20.00 | ml | - | - | |
Trace element solution | 5.00 | ml | - | - | |
Na-acetate | 0.10 | g | 0.1 | 1.219 | |
Agar , if required | 12.00 | g | 12 | 35.679 | |
Distilled water | 1000.00 | ml | - | - | |
1 Adjust pH to 7.5 before autoclaving. Sterilize separately a 10% (w/v) solution of Na2S x 9 H2O; add the following amount of this solution to the medium after autoclaving (= shortly before using the medium). | |||||
neutralized Na2S x 9 H2O solution, 10% (w/v) | 3.00 | ml | - | - | |
2 Note: For small volumes, such as 5 mL agar slants, you can use the respective volume of a sterile neutralized 3% Na2S x 9 H2O solution (e.g., 50µL/5 mL = 0.3g/L) |
573 | THIOTHRIX MEDIUM |