Main sol. 691
Internal Solution-ID: S1439
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KH2PO4 | 1.40 | g | 1.396 | 10.257 | |
NH4Cl | 0.50 | g | 0.499 | 9.32 | |
MgCl2 x 6 H2O | 0.20 | g | 0.199 | 0.981 | |
CaCl2 x 2 H2O | 0.15 | g | 0.15 | 1.017 | |
Trace element solution SL-10 | 1.00 | ml | - | - | |
Selenite-tungstate solution | 1.00 | ml | - | - | |
NaHCO3 | 1.50 | g | 1.496 | 17.802 | |
Na2-DL-malate | 1.60 | g | 1.595 | 8.959 | |
Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients except vitamins, bicarbonate and malate. Adjust pH to 6.5, dispense medium in culture vessels and autoclave. Prior to inoculation add vitamins, bicarbonate and malate from stock solutions sterilized by filtration. Adjust pH of complete medium to 6.7 - 6.9. |
691 | CreDm1 MEDIUM |