Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Casein peptone
(tryptic digest) |
10.00 | g | 10 | - | |
| Meat extract | 10.00 | g | 10 | - | |
| Yeast extract | 5.00 | g | 5 | - | |
| Glucose | 20.00 | g | 20 | 111.012 | |
| Tween 80 | 1.00 | g | 1 | 0.764 | |
| Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
| K2HPO4 | 2.00 | g | 2 | 11.483 | |
| Na-acetate | 5.00 | g | 5 | 60.951 | |
| (NH4)3 citrate | 2.00 | g | 2 | 8.223 | |
| MgSO4 x 7 H2O | 0.20 | g | 0.2 | 0.811 | |
| MnSO4 x H2O | 0.05 | g | 0.05 | 0.296 | |
| Distilled water | 1000.00 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.5 | 2.847 | |
| 1 Cysteine is added after the medium has been boiled and cooled under CO2. Adjust pH to 6.2 - 6.5, distribute under N2 and autoclave. | |||||
Media with this solution
| 11b | MRS MEDIUM (pre-reduced) |