Main sol. 815a
Internal Solution-ID: S1655
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na2SO4 | 3.0 | g | 3 | 21.121 | |
CaCl2 x 2 H2O | 6.0 | g | 6 | 40.812 | |
NaCl | 40.0 | g | 40 | 684.463 | |
KCl | 2.0 | g | 2 | 26.828 | |
NH4Cl | 0.3 | g | 0.3 | 5.609 | |
KH2PO4 | 0.2 | g | 0.2 | 1.47 | |
MgCl2 x 6 H2O | 8.0 | g | 8 | 39.35 | |
SrCl2 x 6 H2O | 0.1 | g | 0.1 | 0.375 | |
Trace element solution SL-11 | 1.0 | ml | - | - | |
MOPS | 3.0 | g | 3 | 14.336 | |
Yeast extract | 1.0 | g | 1 | - | |
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | - | |
Na-pyruvate | 2.5 | g | 2.5 | 22.718 | |
Na2CO3 | 0.5 | g | 0.5 | 4.718 | |
Na2S x 9 H2O | 0.1 | g | 0.1 | 0.416 | |
Distilled water | 1000.0 | ml | - | - | |
1 Dissolve ingredients except pyruvate, bicarbonate and sulfide, adjust pH to 6.0 with NaOH and sparge medium for 30 - 45 min with 100% N2 gas to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Before use add pyruvate (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Final medium pH should be 7.2. | |||||
2 Note: Prior to inoculation 10-20 mg/l sodium dithionite (added from a 5% w/v solution freshly prepared under N2 and filter-sterilized) can be added to the medium to stimulate growth at the beginning. |
815a | DESULFOCELLA HALOPHILA MEDIUM |