Main sol. 913
Internal Solution-ID: S1875
Equipment needed: Filter
Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Artificial sea water | 750.0 | ml | - | - | |
| (NH4)2SO4 | 1.0 | g | 0.999 | 7.56 | |
| KH2PO4 | 0.5 | g | 0.5 | 3.671 | |
| Yeast extract | 2.0 | g | 1.998 | - | |
|
MOPS
(SIGMA) |
5.0 | g | 4.995 | 23.87 | |
| Modified Wolin's mineral solution | 10.0 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
| Wolin's vitamin solution (10x) | 1.0 | ml | - | - | |
| Na2S x 9 H2O | 0.3 | g | 0.3 | 1.248 | |
| Distilled water | 240.0 | ml | - | - | |
| 1 Dissolve ingredients except vitamins and sulfide, adjust pH to 7.0, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials (to 30% of volume) and autoclave. Add vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Vitamins should be sterilized by filtration. Adjust pH of the complete medium to 7.0, if necessary. | |||||
Media with this solution
| 913 | THERMOTOGA PETROPHILA MEDIUM |