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Main sol. 113a

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Internal Solution-ID: S196

Equipment needed: Gassing station Autoclave Filter Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 942 ml - -
Solution B 40 ml - -
Solution C 20 ml - -
Solution D 1 ml - -
Solution E 1 ml - -
1 Solutions A, B, and D are prepared under 100% N2 gas atmosphere and sterilized by autoclaving at 121⁰C for 15 min. Solution C is sterilized by filtration under an atmosphere of 80% N2 and 20% CO2 gas mixture. Solution E is prepared under a 100% N2 gas atmosphere and sterilized by filtration. Appropriate amounts of solutions B to E are added to the sterile solution A in the sequence as indicated. Check the pH of the complete medium and adjust to pH 7.0-7.2, if necessary.
113a SULFURIMONAS HONGKONGENSIS MEDIUM