Back to all solutions

Main sol. 14

Internal Solution-ID: S19

Equipment needed: Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 5.00 g 4.946 -
K2HPO4 10.00 g 9.891 56.789
Trace element solution SL-4 10.00 ml - -
Pyridoxine hydrochloride
(0.1% w/v)
1.00 ml 9.9e-4 0.005
Na-thioglycolate 0.75 g 0.742 6.502
Sodium resazurin
(0.1% w/v)
0.50 ml 4.9e-4 0.002
Na2CO3 1.00 g 0.989 9.332
D-Fructose 5.00 g 4.946 27.451
Agar , for solid medium
optional
15.00 g 14.837 -
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except carbonate and fructose) and sparge medium with 100% N2 gas for at least 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add fructose from a sterile anoxic stock solution prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Adjust pH of complete medium to 8.0.
14 CLOSTRIDIUM FORMICOACETICUM MEDIUM