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Main sol. 1008

Internal Solution-ID: S20

Equipment needed: Hungate tubes Autoclave Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(BD BBL)
2.0 g 2 -
2 1.0 g 1 -
3
(0.1% w/v)
0.5 ml 5.0e-4 0.002
4 1.0 g 1 8.764
5 2.0 g 2 5.843
6 800.0 ml - -
7 200.0 ml 199.641 11081.8
9 , might need strain adjustment 5.0 g/l - -
10 , might need strain adjustment 5.0 g/l - -
Instructions:
  1. Dissolve ingredients except thioglycolate and cellobiose, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add thioglycolate and adjust pH of medium to 7.5 with 10 N KOH. Dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave at 121°C for 15 min. Add cellobiose from a sterile anoxic stock solution prepared under 100% N2 gas atmosphere and sterilized by filtration.
  2. Note: Bottled seawater from Biomaris GmbH can be used instead of filtered seawater.
1008 SEDIMINISPIROCHAETA MEDIUM