Clarified rumen fluid
Internal Solution-ID: S2618
This solution is used in 29 media and was first defined in medium 1310.
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| 1 Rumen fluid from cow or sheep (obtained from fistulated animals or abattoir refuse) is filtered through muslin, autoclaved at 121°C for 15 min and then centrifuged at 27,000 g for 20 min. The supernatant is made anoxic by sparging with 100% N2 gas for 15 min, dispensed under same gas atmosphere into anoxic serum vials to 30% of volume and then stored frozen at -20°C. |
Media with this solution
| 78c | CHOPPED MEAT MEDIUM FOR FRETIBACTERIUM SP. |
| 119a | METHANOBREVIBACTER MEDIUM |
| 140 | RUMINICLOSTRIDIUM MEDIUM |
| 159 | TREPONEMA MEDIUM |
| 161 | METHANOFOLLIS MEDIUM |
| 201 | DESULFONEMA LIMICOLA MEDIUM |
| 212a | TREPONEMA RECTALE MEDIUM |
| 212 | SYNTHROPHOMONAS MEDIUM |
| 213 | SYNTHROPHOMONAS MEDIUM (SULFATE FREE) |
| 275 | TREPONEMA SUCCINIFACIENS MEDIUM |
| 320 | CLOSTRIDIUM CELLULOVORANS MEDIUM |
| 321 | METHANOCORPUSCULUM AGGREGANS MEDIUM |
| 322 | METHANOSPHAERA MEDIUM |
| 336 | OXOBACTER PFENNIGII MEDIUM |
| 337 | SYNTROPHOCOCCUS SUCROMUTANS MEDIUM |
| 346 | DESULFALLAS MEDIUM |
| 347 | SYNTROPHOMONAS SAPOVORANS MEDIUM |
| 397 | FERULATE MEDIUM |
| 407 | SYNTROPHOMONAS SD2 MEDIUM |
| 523 | CLOSTRIDIUM CHARTATABIDUM MEDIUM |
| 666a | CELLULOSILYTICUM MEDIUM |
| 666 | SUCCINICLASTICUM MEDIUM |
| 666b | METHANOMICROBIUM MOBILE MEDIUM |
| 704 | BUTYRIVIBRIO SP. MEDIUM |
| 734a | METHANOBREVIBACTER CUTICULARIS MEDIUM |
| 734 | METHANOBREVIBACTER CURVATUS MEDIUM |
| 912 | TREPONEMA R8 MEDIUM |
| 915 | BLAUTIA MEDIUM |
| 1310 | BUTYRIVIBRIO M2 MEDIUM |