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Main sol. 150a

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Internal Solution-ID: S265

Equipment needed: Autoclave Filter Water bath
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 3.00 g 2.97 22.478
K2HPO4 x 3 H2O 0.50 g 0.495 2.842
MgSO4 x 7 H2O 0.50 g 0.495 2.009
KCl 0.10 g 0.099 1.328
Ca(NO3)2 x 4 H2O 0.02 g 0.02 0.084
Trace element solution 10.00 ml - -
Sulfur
(powdered) 		5.00 g 4.951 154.365
Distilled water 1000.00 ml - -
1 Dissolve ingredients except trace elements and sulfur. Adjust pH with 6 N H2SO4 to 2.5, dispense into suitable cultivation vessels and autoclave. After autoclaving add filter-sterilized trace elements solution (see below) and sterile sulfur powder. For sterilization place the sulfur in screw-capped tubes or bottles, wet with a few drops of water and heat for 3 hours to 90 - 100°C in a water bath on each of three successive days. Before use, aseptically layer the sulfur onto the surface of the autoclaved liquid basal medium.
150a ACIDITHIOBACILLUS CALDUS MEDIUM