Main sol. 150a
Internal Solution-ID: S265
| Zutat | Menge | Einheit | Konz. [g/L] | Konz. [mM] | |
|---|---|---|---|---|---|
| (NH4)2SO4 | 3.00 | g | 3 | 22.703 | |
| K2HPO4 x 3 H2O | 0.50 | g | 0.5 | 2.871 | |
| MgSO4 x 7 H2O | 0.50 | g | 0.5 | 2.029 | |
| KCl | 0.10 | g | 0.1 | 1.341 | |
| Ca(NO3)2 | 0.01 | g | 0.01 | 0.061 | |
| Trace element solution | 10.00 | ml | - | - | |
|
Sulfur
(powdered) |
5.00 | g | 5 | 155.909 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients except trace elements and sulfur. Adjust pH with 6 N H2SO4 to 2.5, dispense into suitable cultivation vessels and autoclave. After autoclaving add filter-sterilized trace elements solution (see below) and sterile sulfur powder (sterilized by steaming for 3 hourson each of 3 successive days). | |||||
| 150a | ACIDITHIOBACILLUS CALDUS MEDIUM |