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Main sol. 150a

Internal Solution-ID: S265

Benötigtes Equipment: Autoclave Filter Water bath
Zutat Menge Einheit Konz. [g/L] Konz. [mM]
(NH4)2SO4 3.00 g 3 22.703
K2HPO4 x 3 H2O 0.50 g 0.5 2.871
MgSO4 x 7 H2O 0.50 g 0.5 2.029
KCl 0.10 g 0.1 1.341
Ca(NO3)2 0.01 g 0.01 0.061
Trace element solution 10.00 ml - -
Sulfur
(powdered)
5.00 g 5 155.909
Distilled water 1000.00 ml - -
1 Dissolve ingredients except trace elements and sulfur. Adjust pH with 6 N H2SO4 to 2.5, dispense into suitable cultivation vessels and autoclave. After autoclaving add filter-sterilized trace elements solution (see below) and sterile sulfur powder (sterilized by steaming for 3 hourson each of 3 successive days).
150a ACIDITHIOBACILLUS CALDUS MEDIUM