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Wolfe's Mineral Solution

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Internal Solution-ID: S2680

Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 3.00 g 3 24.923
MnSO4 0.50 g 0.5 3.311
NaCl 1.00 g 1 17.112
FeSO4 0.10 g 0.1 0.658
CaCl2 0.10 g 0.1 0.901
CoCl2 0.10 g 0.1 0.77
ZnSO4 0.10 g 0.1 0.62
CuSO4 0.01 g 0.01 0.063
AlK(SO4)2 0.01 g 0.01 0.039
H3BO3 0.01 g 0.01 0.162
Na2MoO4 0.01 g 0.01 0.049
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals.
2 Adjust to pH 8.0 with KOH and sterilize by autoclaving.
1337 METHYLOPHAGA MEDIUM