Main sol. 1696
Internal Solution-ID: S3514
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 20.0 | g | 20 | 342.231 | |
| Na2CO3 | 50.0 | g | 50 | 471.752 | |
| NaHCO3 | 60.0 | g | 60 | 714.226 | |
| KH2PO4 | 0.2 | g | 0.2 | 1.47 | |
| MgCl2 x 6 H2O | 0.1 | g | 0.1 | 0.492 | |
| Trace element solution | 1.0 | ml | - | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Final pH 9.0 - 9.5 | |||||
| 2 Prepare and boil the medium in the absence NaHCO3, Na2CO3.Cool under a stream of nitrogen and add the remaining components. Dispense into tubes or bottle fitted with rubber stoppers and autoclave. | |||||
Media with this solution
| 1696 | MEDIUM for Z-1701, Z-1702 |