Main sol. 1721
Internal Solution-ID: S3559
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaNO3 | 1 | g | 1 | 11.765 | |
NaHCO3 | 1 | g | 1 | 11.904 | |
Na2S2O3 x 5 H2O | 1 | g | 1 | 4.029 | |
Sulfur
(powdered) |
10 | g | 10 | 311.818 | |
Synthetic seawater
(MJ) |
1000 | ml | - | - | |
1 Prepare the medium under an atmosphere of H2/CO2 (80:20) without adding NaNO3, Na2S2O3 x 5 H20 and NaHCO3 in serum bottles and seal the serum tubes with butyl rubber stoppers. Steam medium for 3 hours on each of 3 successive days. To the sterile medium add, from filter sterilised stock solutions, NaNO3, Na2S2O3 x 5 H20 and NaHCO3. | |||||
2 Increase the 80% H2 + 20% CO2 gas phase pressure to 0.3 MPa. The final pH is 7.0. |
1721 | MMJHS MEDIUM |