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Main sol. 1721

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Internal Solution-ID: S3559

Compound Amount Unit Conc. [g/L] Conc. [mM]
NaNO3 1 g 1 11.765
NaHCO3 1 g 1 11.904
Na2S2O3 x 5 H2O 1 g 1 4.029
Sulfur
(powdered) 		10 g 10 311.818
Synthetic seawater
(MJ) 		1000 ml - -
1 Prepare the medium under an atmosphere of H2/CO2 (80:20) without adding NaNO3, Na2S2O3 x 5 H20 and NaHCO3 in serum bottles and seal the serum tubes with butyl rubber stoppers. Steam medium for 3 hours on each of 3 successive days. To the sterile medium add, from filter sterilised stock solutions, NaNO3, Na2S2O3 x 5 H20 and NaHCO3.
2 Increase the 80% H2 + 20% CO2 gas phase pressure to 0.3 MPa. The final pH is 7.0.
1721 MMJHS MEDIUM