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Main sol. J434

Internal Solution-ID: S4180

1 Use Medium No. 284 supplemented with final 1.0 ml Trace vitamins solution in Solution A (per 900 ml), and with 7.2 g sucrose in Solution B (per 100 ml) instead of sodium pyruvate. Otherwise, prepare the medium as described in the recipe of Medium No. 284.
2 Comment: For strains JCM 12577 and JCM 12578, incubate with a 10% inoculum.
J434 ANAEROLINEA MEDIUM