Solution A
Internal Solution-ID: S4390
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KH2PO4 | 0.3 | g | 0.333 | 2.444 | |
K2HPO4 | 0.3 | g | 0.333 | 1.91 | |
NH4Cl | 1.0 | g | 1.109 | 20.726 | |
Na2SO4 | 3.0 | g | 3.326 | 23.416 | |
NaCl | 23.0 | g | 25.499 | 436.326 | |
KCl | 0.1 | g | 0.111 | 1.487 | |
CaCl2 | 0.1 | g | 0.111 | 0.999 | |
Yeast extract
(BD-Difco) |
0.1 | g | 0.111 | - | |
Trace element solution | 1.0 | ml | - | - | |
L-Cysteine HCl x H2O | 0.5 | g | 0.554 | - | |
Resazurin | 1.0 | mg | 0.001 | 0.005 | |
Distilled water | 900.0 | ml | - | - | |
1 To complete the medium, add the following stock solutions [Filter-sterilize NaHCO3 solution and store under a N2-CO2 (4:1, v/v) gas mixture. The other solutions are autoclaved and stored anaerobically under a N2 atmosphere] to the medium (per 4.5 ml): | |||||
Sodium lactate
(1 M) |
0.1 | ml | - | - | |
MgCl2 x 6 H2O
(15%) |
0.1 | ml | - | - | |
NaHCO3
(5%) |
0.2 | ml | - | - | |
Na2S x 9 H2O
(2%) |
0.1 | ml | - | - | |
2 Mix ingredients and adjust pH to 7.2. Distribute 0.9 volume of the medium into tubes (e.g., 4.5 ml of the medium in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave at 110C for 45 min. |
J565 | DESULFOVIBRIO HEB233 MEDIUM |