Main sol. J607
Internal Solution-ID: S4465
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 100.0 | g | 96.062 | 1643.76 | |
MgCl2 x 6 H2O | 5.0 | g | 4.803 | 23.625 | |
MgSO4 x 7 H2O | 9.5 | g | 9.126 | 37.025 | |
KCl | 5.0 | g | 4.803 | 64.428 | |
CaCl2 x 2 H2O | 0.2 | g | 0.192 | 1.307 | |
(NH4)2SO4 | 0.1 | g | 0.096 | 0.727 | |
KNO3 | 0.1 | g | 0.096 | 0.95 | |
Peptone | 5.0 | g | 4.803 | - | |
Yeast extract | 1.0 | g | 0.961 | - | |
Distilled water | 1000.0 | ml | - | - | |
1 Adjust pH to 7.0. For preparation of solid medium, add 15.0 g/L agar. Autoclave. After cooling to 50C, add the following solutions to the medium (autoclaved or *filter-sterilized): | |||||
Metal solution | 20.0 | ml | - | - | |
Phosphate solution | 20.0 | ml | - | - | |
Trace vitamins
(*) |
1.0 | ml | - | - |
J607 | HALOMONAS SUBGLACIESCOLA MEDIUM |