Main sol. J909
Internal Solution-ID: S4911
Equipment needed: Filter
Autoclave
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.3 | g | 0.295 | 2.166 | |
| K2HPO4 | 0.3 | g | 0.295 | 1.692 | |
| NH4Cl | 1.0 | g | 0.982 | 18.365 | |
| NaCl | 23.0 | g | 22.593 | 386.607 | |
| KCl | 0.1 | g | 0.098 | 1.318 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.098 | 0.668 | |
| Trace minerals | 10.0 | ml | - | - | |
| Yeast extract | 0.5 | g | 0.491 | - | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.491 | - | |
| Resazurin | 1.0 | mg | 9.8e-4 | 0.004 | |
| Distilled water | 940.0 | ml | - | - | |
| 1 Mix components thoroughly and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions from anaerobic stocks (autoclaved or *filter-sterilized): | |||||
|
NaHCO3
(*, 8%) |
25.0 | ml | - | - | |
|
MgCl2 x 6 H2O
(10%) |
30.0 | ml | - | - | |
|
Glucose
(1 M) |
5.0 | ml | - | - | |
| 2 Aseptically distribute the medium into culture vessels under a stream of N2-CO2 (4:1, v/v) and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solution from an anaerobic stock (autoclave and store under a N2 atmosphere): | |||||
|
Na2S x 9 H2O
(5%) |
8.0 | ml | - | - | |
Media with this solution
| J909 | IRD MARINE CLOSTRIDA MEDIUM |