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10 x Korthof's basal medium

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Internal Solution-ID: S4968

Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 7.0 g 15.556 266.18
Na2HPO4 4.4 g 9.778 68.878
Polypeptone
(BD-BBL) 		4.0 g 8.889 -
KH2PO4 1.2 g 2.667 19.596
NaHCO3 0.1 g 0.222 2.645
KCl 0.2 g 0.444 5.962
CaCl2 x 2 H2O 0.2 g 0.444 3.023
Distilled water 450.0 ml - -
1 Mix ingredients except CaCl2 and autoclave. Dissolve CaCl2 in 50 ml distilled water and filter-sterilize. After autoclaving, allow to cool, add sterile CaCl2 solution to the medium.
J946 KORTHOF'S MEDIUM