Main sol. J954
Internal Solution-ID: S4976
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MDS salt water | 767.00 | ml | - | - | |
| Yeast extract | 1.00 | g | 0.993 | - | |
|
Fish peptone
(Fluka) |
0.25 | g | 0.248 | - | |
| Sodium formate | 0.25 | g | 0.248 | 3.651 | |
| Sodium acetate | 0.25 | g | 0.248 | 3.026 | |
| Sodium lactate | 0.25 | g | 0.248 | 2.215 | |
| Sodium pyruvate | 0.25 | g | 0.248 | 2.256 | |
| Distilled water | 233.00 | ml | - | - | |
| 1 Mix components thoroughly and adjust pH to 7.0. For preparation of solid medium, add 20.0 g/L agar. (if necessary, use washed agar as described in the recipe of Medium No. 574). Autoclave and, after cooling, add the following solutions (*filter-sterilized or autoclaved) to the medium. | |||||
|
NH4Cl
(*, 1 M) |
5.00 | ml | 0.267 | 5 | |
| Potassium phosphate buffer | 2.00 | ml | - | - | |
Media with this solution
| J954 | NEUTRAL OLIGOTROPHIC HALOARCHAEAL MEDIUM NO.3 |