Main sol. J1105
Internal Solution-ID: S5193
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Yeast extract | 10 | g | 1000 | - | |
| Trisodium citrate x 3 H2O | 3 | g | 300 | - | |
| MgSO4 x 7 H2O | 1 | g | 100 | 405.719 | |
| KCl | 3 | g | 300 | 4024.14 | |
| NaCl | 100 | g | 10000 | 171116 | |
| 1 Add components to distilled water and bring volume to 1.0 L. For preparation of solid medium, add 20.0 g/L agar. After autoclaving, add the following solution (autoclaved): | |||||
|
Na2CO3
(10%) |
10 | ml | - | - | |
| 2 Readjust pH to 8.0, if necessary. | |||||
Media with this solution
| J1105 | VIRGIBACILLUS MERV MEDIUM |